Objective In order to provide data for the formulation of maximum residue limitsassess (MRL) in wolfberry, the pesticide residues and the cumulative risk of pyrethroid were studied. Methods Three hundred and thirty-two kinds of pesticides including 15 pyrethroids in 40 samples which collected from different place were determined. Acute and chronic cumulative risk assessment were calculated by using hazard index approach. Results This method was appropriate to determine the pyrethroid residues in wolfberry by validation of the method. The result showed that the detecion rate of 4 pyrethroids was over 25%, including cypermethrin, fenvalerate, enpropathrin and cyhalothrin. The acute and chronic cumulative risk of pyrethroids in wolfberry was 0.497 and 0.016. Conclusion The cumulative exposure of 4 pyrethroids in wolfberry was small, but continuous monitoring should be maintained because of its high detection rate, and MRL also should be considered.