Objective For implementation of labeling regulations, an event-specific real-time polymerase chain reaction(PCR) method for the detection of genetically modified AquAdvantage salmon was established in this study. Methods Primers and TaqMan probe were designed based on the event-specific sequence of AquAdvantage salmon. The specificity, sensitivity and repeatability of the developed method were examined, respectively. Results The specificity test of this method showed it was specific to AquAdvantage salmon. The 600 000-60 copies range showed a good linear relationship with Ct values, and its linear regression equation was y=-3.2194x+40.805(R²=0.997). The limit of quantification(LOQ) was 60 copies and the repeatability was good. Conclusion This event-specific real-time PCR method was suitable for the identification of genetically modified AquAdvantage salmon.