Abstract:Objective To establish a method for the determination of 4-chlorophenoxyacetic acid, 2,4-dichlor-ophenoxyacetic acid, naphthylacetic acid, indole acetic acid and indolybutyric acid in fruits by QuEChERS with gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods Five plant growth regulators in fruits were extracted by methylene chloride, purified by QuEChERS, and were identified and quantified by GC-MS/MS after derivatization from the purified samples. Results The linear correlation coefficients of each linear regression equation for the five plant growth regulators were over 0.999 and the relative standard deviations (n=6) were between 0.480% and 14.8%. The recoveries of the method were between 63.4% and 107% at the spiked levels of 10.0-200 μg/kg. Conclusion The method was rapid and accurate, and could meet the requirement for the detection of the five kinds of plant growth regulators in fruits.