Objective To establish a method for the determination of pesticide residues in health food made from Chinese herbal medicines by dispersive solid phase extraction and dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry. Methods The samples were ultrasonic extracted by acetonitrile containing 0.5% formic acid. The supernatant was purified by dispersive solid phase extraction (d-SPE) extraction tube with anhydrous magnesium sulfate, N-propyl ethylenediamine (PSA) and C18, and then the target compounds were extracted and concentrated by the method of dispersive liquid-liquid microextraction (DLLME). Results Under optimal conditions, the linear range was 0.05-1.00 μg/ml, the linear correlation coefficient was above 0.999, the limit of detection was 0.3-3.0 μg/kg, the average recoveries of 29 kinds of pesticides were between 70.3% and 107.5%, and the relative standard deviation was less than 10%. Conclusion The method was simple, rapid, accurate and sensitive, and it could be used for the detection of organophosphorus pesticide residues in health food made from Chinese herbal medicines.