Objective To study the molecular biological characteristics of food poisoning Salmonella isolates, and analyze the homology and resistance relationship between strains, which could provide scientific basis for prevention and control of foodborne disease and clinical treatment. Methods Totally 40 strains of Salmonella isolates were tested for susceptibility and molecular typing by pulsed field electrophoresis (PFGE). BioNumeries software was used to analyze the epidemiological relationships between strains. Results All the strains could be divided into 11 types by Xba I enzyme digestion, and drug sensitivity showed multiple drug resistance. Conclusion Foodborne Salmonella was highly sporadic and multidrug resistant was found in this region. The resistance of Salmonella was obvious, especially predominance serum enteritis, which suggested that it was necessary to strengthen the surveillance of Salmonella resistance.