Objective To investigate the distribution of Salmonella serotypes in food and feed from the import and export of Beijing port during 2004 to 2012. Methods In this study, 65 Salmonella strains isolated from food and feed were identified. The serotyping was performed with 13 reference strains. Results Four Salmonella isolate strains could not be identified to the serovar. The rest of 61 Salmonella isolated strains belonged to 9 serovars and 38 serotypes. The group B and group C1 were the major serovas. The ratio was 29.2%(19/65) and 26.2%(17/65) respectively. Salmonella agona and Salmonella enteritidis took the proportion of 10.8%(7/65)and 3.1%(2/65)respectively. It was the first time a strain of Salmonella stratford was detected in this laboratory. Conclusion There was a variety of serogroups and serotypes of Salmonella in the import and export food and feed from Beijing port. Salmonella serotypes surveillance was important for control and prevention of the foodborne disease in the region.