Objective To investigate a poisoning caused by wild mushrooms and to identify the toxin in these mushrooms. Methods Epidemiological investigation, blood test and mushroom toxin were analyzed. Results This incident was taken place in one family, and all family members were dead. Multiple organ damage was observed in all patients; amatoxins and virotoxins were detected in both mushrooms and the soup, but were not detected in blood samples because of dialysis. Conclusion The incident was caused by wild mushrooms and public education shoud be strenthened to urge people to avoid eating wild mushrooms and go to the hospital immediately if poisoning takes place.