Objective An analytical method for rapid screening and confirmation of multiple pesticide residues in vegetable and fruit was established according to GB 2763-2014 national food safety standard-maximum residue limits for pesticides in food, using ultra performance liquid chromatography-Q-time of flight mass spectrometry (UPLC-Q-TOF-MS). Methods Pesticide residues in vegetable and fruit were screened by comparing the accurate mass, isotope distribution and abundance in the accurate mass database, and confirmed by comparing with the spectra in the spectra library or by the spectral analysis method. Results The method was verified by spiked samples, and all pesticides were detected at the addition level of 10.0 and 50.0 μg/kg. The recovery of 90% of the pesticides was in the range of 70%-120%, with relative standard deviation (RSD) ≤20% (n=5). The method was applied to screen pesticides in 30 samples of vegetables and fruits. Twenty-seven pesticides were found and 2 samples exceeded the limit. Conclusion The method was sensitive, accurate and reproducible. Hundreds of pesticides in GB 2763-2014 could be screened and confirmed in a short period of time even without standard substance. It could provide an effective method for food safety control.