Detection and comparison of 10 human milk oligosaccharides during lactational stage
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(1.Beijing Research Institute for Nutritional Resources,Beijing 100069,China;2.Nestle Research Center Beijing Hub,Beijing 100095,China;3.National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention,Beijing 100050,China;4.Beijing Bonnysci Co., Ltd,Beijing 100069,China)

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    Abstract:

    Objective To detect 10 kinds of human milk oligosaccharides (HMOS) and compare their amounts during lactational stage. Methods Breast milk samples in different stages of lactation as colostrum (day 0-7 postpartum), transitional milk (day 8-15 postpartum), and mature milk (day 16-180 postpartum) were collected and 10 HMOS in those samples were detected and quantified by ultra high performance liquid chromatography-fluorescence detection after fluorescence labeling by using standard curves. Correlations between HMOS and lactation day were conducted by Person correlation analysis method , while the differences among three stages were calculated by ANOVA test. Results Ten HMOS were successfully separated and quantified under chosen chromatographic parameters. 2′FL,3′SL, 6′SL, LNT, LNnT and LNFP-I were negatively correlated and 3′FL was positively correlated with lactation days. They were different in three lactational stages (P<0.05), while PI, LNFP-V and LNnFP-V showed no correlation and difference (P>0.05). Conclusion The amount of HMOS changed during lactational stages. Seven HMOS were correlated with lactation days and different in three lactational stages (P<0.05).

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ZHU Jing, SHI Yu-jie, WU Li-fang, SUN He-nan, WANG Xin, WANG Zhu, GUAN Lei, YU Kai, YANG Yue-xin. Detection and comparison of 10 human milk oligosaccharides during lactational stage[J].中国食品卫生杂志,2017,29(4):417-422.

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  • Received:April 25,2017
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  • Online: August 22,2017
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