To investigate the attitude of food industries on GB 14880-2012 Standard on the Use of Food Nutritional Fortification Substances and the impact of its implementation on food industries. Methods A questionnaire survey was designed and carried out in 25 provinces on food industries in 2014, and 686 valid questionnaires were collected. Descriptive analysis and chi square test were used for statistical analysis. Results The awareness rate of food industries for the standard was 87.61% (601/686), the average score of maneuverability was 4.325 points at a 5 points scale, and the score of rationality was 4.293 points. 92.15% (552/599) of the food industries had taken different actions to implement the new standard, and it had played a positive role, however it also increased the cost of inspection for food industries. Conclusion The awareness rate of food industries was high, and food industries had high satisfaction score for maneuverability and rationality on GB 14880-2012. The new standard played a positive role for the food industries. However, the implementation of new standards also brought inspection cost for food industries.