To investigate the association between the multilocus sequence tying(MLST)of Listeria monocytogenes and its antibiotic susceptibility, and to determine the presence of some clonal strains with high pathogenic potential.Methods Susceptibility tests were performed through the Kirby-Bauer (K-B) method and E-test method with fourteen antibiotics; and then 50 Listeria monocytogenes strains were genotyped by MLST. Results The antibiotic resistant rate of Listeria monocytogenes was 22.00%(11/50), and multi-resistant strains appeared during the tests. The 50 Listeria monocytogenes strains were divided into 12 types based on MLST typing with ST9 and ST121 was being the dominant types. Conclusion Specific STs could transfer to particular strains during food production processes. The resistant strains were found in both foodborne and human source Listeria monocytogenes. There was a possibility of the transmission of antibiotic-resistant genes, thus the monitoring of potentially pathogenic ST-type was quite important to reduce Listeria monocytogenes.