Survey on the contamination of Norovirus in shellfish, vegetable, fruit and ready-to-eat seafood from markets in Beijing
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(1.National Institute of Food and Drug Control,Beijing 100050,China;2.Jilin Province Inspection Center for Food and Drug,Jilin Changchun 130012,China;3.Gansu Province Food Inspection Institute,Gansu Lanzhou 730030,China)

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    Abstract:

    To investigate the contamination of Norovirus in shellfish (oysters, mussels, scallops, clams, clam), vegetables (seedling vegetables, lettuce), berries (strawberries, blueberries), ready-to-eat seafood (salmon, shrimp ) from markets in Beijing.Methods Pretreatment methods for shellfish, vegetables and berries samples were referred to ISO/TS 15216-1; pretreatment method for shrimp and shellfish was referred to shellfish method; pretreatment method for salmon was referred to improved vegetables method. Viral RNA extraction was referred to Roche High pure viral RNA Kit K methods. Real-time reverse transcription-polymerase chain reaction testing and GI-GII typing were carried out by Roche LightMix noroviurus GI-GII in Lightcycler 480 II; Norovirus positive samples was amplified using the prime of JV12, JV13 and the amplified DNA fragments were sequenced for subtyping. Results 478 shellfish (40 positive), 62 vegetables (1 positive), 84 berries (0 positive) and 51 raw salmon (1 positive) were detected. 35 positive samples were successfully amplified by sequence analysis. 27 samples were GII.17,1 sample was GII.12, and 1 sample was Hawaii.calicivirus.Conclusion Through the norovirus monitoring in Beijing market, shellfish presented obvious seasonal distribution and higher detection rate which suggested that the shellfish was the main sources of Norovirus in food.

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LUO Hai-peng, GAO Fei, YU Hai-yao, TONG Wei-jian, REN Xiu, YU Wen, CUI Sheng-hui. Survey on the contamination of Norovirus in shellfish, vegetable, fruit and ready-to-eat seafood from markets in Beijing[J].中国食品卫生杂志,2017,29(2):218-222.

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  • Received:February 15,2017
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  • Online: May 03,2017
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