To establish a method for the determination of inorganic and organic selenium in food by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC/ICP-MS).Methods Inorganic selenium was extracted by ultrapure water. Dialysis effect was used to remove sample matrix, inorganic selenium was separated and detected by anion exchange chromatography-inductively coupled plasma mass spectrometry. Results The detection limit of Se(IV) and Se(VI) were 6.67 and 9.95 μg/kg, the recoveries were 70.2%-108.1% and 73.2%-116.9%.Conclusion This method could be used for the determination of inorganic selenium and organic selenium in selenium-enriched food.