Blood glucose meter is an electronic device for determining the concentration of glucose in blood. Due to its advantage of rapid, portable, low cost, and simple operation, it has been widely available for clinical diagnosis. However, the test subject is only glucose. Thus, there is much demand to explore new method based on blood glucose meter for the determination of a wide range of biomarkers, nucleic acid, and other analytical targets. Recently, coupling target recognition elements with signal transduction and amplification strategies, various analytical targets were determined. This paper briefly reviews the development of blood glucose meter and describes its potential application in food analysis in detail. The prospect of future development of glucose meter is also discussed.