To find out the cause and guide the clinical treatment so as to provide the basis for the prevention and cure of similar food poisoning caused by Clostridium botulinum.Methods According to the principles of Clostridium botulinum food poisoning management, treatment plan was determined and applied following clinical diagnosis, laboratory testing and antitoxic serum intervention through the emergency procurement system for specific medicine. Results Two patients were identified with one hospitalized and there was no death in this case. The hospitalized patient was treated with antitoxins and mechanic ventilation and cured in 25 days.Conclusion The poisoning case was caused by intake of home-made food contaminated by Clostridium botulinum. It is suggested that public education in food safety should be strengthened.