To explore the temporal-spatial distribution characteristics of the paralytic shellfish poison in Shenzhen City, in order to determine the key areas of pollution and provide the basis for early alert.Methods The paralytic shellfish poison monitor data from three monitoring sites in Shenzhen (Xiexia Bay, Dongyong and Pearl Island) in 2015 were analyzed using the radial basis function interpolation and spatial-temporal scan, and the general distribution and spatial-temporal clustering characteristics were preliminarily explored. Results According to radial basis function interpolation, the content of paralytic shellfish poison in Xiexia Bay was relatively high compared to Dongyong and Pearl Island. There were obvious differences in the distribution characteristics of the paralytic shellfish poison in Xiexia Bay, Dongyong and Pearl Island. Spatial-temporal scan presented space-time aggregation, from January 2015 to June 2015, the Xiexia Bay was most likely to be a aggregation region (LLR=7.72, RR=5.59, P=0.000 83＜0.001). Conclusion The spatial distribution regularity and spatial-temporal clustering characteristics of the paralytic shellfish poison could be obtained by the methods of radial basis function interpolation and spatial-temporal scan.