Rapid determination of β-carotene in compound vitamin tablets by reverse-phase high performance liquid chromatography
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(By-Health Company Limited,Guangdong Zhuhai 519040,China)

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    Abstract:

    To establish a method for determination of microencapsulated β-carotene in compound vitamin tablets by reverse-phase high performance liquid chromatography.Methods Microcapsules in the samples were destructed with 0.1 mol/L sodium carbonate solution, and ultrasonically extracted with methanol-acetonitrile(7∶3,V/V)using Agilent SB-C8 column. Methanol-acetonitrile(9∶1,V/V)was the mobile phase, and the detection wavelength was 448 nm. Results β-carotene was completely separated in the chromatographic conditions. The linear range was 0.41-6.20 μg/ml, the average recovery was 99.4%-103.3%, and the RSD was below 2.0%.Conclusion The method was fast and simple, the precision, recovery, and repeatability met the requirements, and could determine microencapsulated β-carotene in compound vitamin tablets.

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SU Zhao-Lun, LI Dan, ZHANG Xi-Jin, YOU Jing-Shui. Rapid determination of β-carotene in compound vitamin tablets by reverse-phase high performance liquid chromatography[J].中国食品卫生杂志,2017,29(1):56-59.

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  • Received:July 18,2016
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  • Online: March 15,2017
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