To express the Norovirus GII.3 capsid protein by gene recombination. Methods The Norovirus GII.3 capsid protein gene was modified and inserted into the pHTA plasmid. The recombinant plasmid was transformed into the MAX Efficiency DH10BacTM competent cell after sequencing and the expression plasmid was obtained. The plasmid was transformed into SF9 cell, and the recombinant capsid protein was expressed. The recombinant capsid protein was purified by Ni-NTA His affinity chromatography purification column followed by identification with sodium dodecyl sufate polyacrylamide gel electrophoresis and Western blot. Results The recombinant capsid protein of about 60 kDa was expressed. It had good immunogenicity, which was verified by animal experiments. The recombinant capsid protein made it possible to prepare monoclonal antibody against Norovirus GII.3 and to develop the immunology detection method.Conclusion The Norovirus GII.3 capsid protein expression plasmid was constructed and the recombinant capsid protein was expressed.