Home > Archive>Volume 29, Issue 1, 2017 >5-8. DOI:10.13590/j.cjfh.2017.01.002
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Identification, virulence gene determination and antibiotic resistance of Listeria monocytogenes in fresh shellfish from shellfish beds
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(1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture, P. R. China; Laboratory of Quality and Safety Risk Assessment for aquatic products (Qingdao), Ministry of Agriculture, P. R. China,Shandong Qingdao 266071,China;2.Zoneco Group Company Limited, Liaoning Dalian 116001,China;3.Shanghai Ocean University,Shanghai 201306,China)

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    Abstract:

    To study the contamination of Listeria monocytogenes in fresh shellfish collected from shellfish beds, and to analyze their virulence genes and antibiotic resistance.Methods Presumptive Listeria monocytogenes were isolated from 200 shellfish samples using the national food safety standard, Food Microbiological Examination:Lisiteria monocytogenes (GB/T 4789.30-2010). The antimicrobial resistance was performed by Kirby-Bauer method and the virulence genes were determined by PCR amplification. Results Among 4 (2.0%) Listeria monocytogenes isolates, 2 strains lacked inlB gene or mpl gene respectively. All the 4 strains were susceptible to the first-line therapeutic antibiotics including ampicillin and gentamicin. Two strains were resistant to tetracycline and trimethoprim-sulfamethoxazol and 1 strain was resistant to trimethoprim-sulfamethoxazol and ofloxacin. Conclusion Samples collected from the shellfish beds were contaminated with Listeria monocytogenes, of which some isolates were lack of virulence genes and were resistant to several important antibiotics. It was necessary to strengthen the contamination and drug resistance monitoring of Listeria monocytogenes in fresh primary aquatic products.

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YAO Lin, JIANG Yan-hua, LI Feng-ling, ZHANG Yuan, ZHANG Qi, ZHU Wen-jia, GUO Ying-ying, WANG Lian-zhu, ZHAI Yu-xiu. Identification, virulence gene determination and antibiotic resistance of Listeria monocytogenes in fresh shellfish from shellfish beds[J].中国食品卫生杂志,2017,29(1):5-8.

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History
  • Received:November 09,2016
  • Online: March 15,2017
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