To investigate the contamination of foodborne pathogenic bacteria in raw food in Pudong New Area, Shanghai.Methods In 2015, nine kinds of raw food were collected for the detection of pathogenic bacteria, and the isolates were serotyped. Results A total of 41.10% (494/1 202) food was contaminated, and 587 pathogenic bacteria were isolated. Vibrio parahaemolyticus (194), Aeromonas (134) and Listeria monocytogenes (131) were the most prevalent pathogens. The highest detection rate was in the second quarter (47.85%) and in shrimp (64.71%). The distribution of major pathogenic bacteria in foods was different. The dominant serogroup/serotype/species were O∶1-O∶5 (Vibrio parahaemolyticus,81.96%), O∶8 and O∶5 (Yersinia enterocolitica,50.00%), A.veronii (Aeromonas,67.91%), C.jejuni and C.coli (Campylobacter,89.29%). The distribution of Salmonella serotypes was various.Conclusion The contamination of foodborne pathogenic bacteria in raw food was serious in Pudong New Area, Shanghai. The distribution of pathogens was diverse and seasonal, and co-contamination occurred.