To develop a rapid method for determination of chloramphenicol and metronidazole in chicken and egg by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and provide analytical methodology for chicken and egg in Ningxia.Methods Samples of chicken and eggs were protein precipitated with acetonitrile, then extracted by ethyl acetate and acetonitrile, and cleaned up by Oasis MCX column. Chloramphenicol and metronidazole were separated on ACQUITY BEH C₁₈ chromatographic column (2.1 mm×100 mm, 1.7 μm) with mobile phase of acetonitrile-water on gradient elution, then determined and quantified by mass spectrometry. Results Average recoveries of chloramphenicol and metronidazole at three spiked levels were 96.3%-110.5% with relative standard deviations less than 7.0%. The limit of detection (LOD) and limit of quantification (LOQ) for both chloramphenicol and metronidazole were 0.1 μg/kg and 0.3 μg/kg.Conclusion The method was accurate, reliable, and could be used as a quality control method for chloramphenicol and metronidazole in chicken and egg.