To study the effect of boxthorn seed oil and flaxseed oil complex on mice immunity.Methods Chose 200 institute of cancer research(ICR)mice and divided into five groups, and 40 mice in each group were randomly divided into 4 subgroups according to body weight, including three different doses(0.25,0.50,1.50 g/kg BW)and the control. After gavage administration of 30 days, body weight, immune organ-body ratios, cell immunity, humoral immunity, mononuclear macrophage phagocytosis and natural killer(NK)cell activity index were measured. Results Compared with the control group, the medium and high dose groups had significantly increased delayed-type hypersensitivity(DTH)and NK cell activity(P<0.05). The high dose groups also showed a significant increase in half hemolytic content(HC₅₀)and antibody generating cell(P<0.05). There were no significant changes in body weight gains, immune organ-body ratios, mononuclear macrophage phagocytosis and lymphocyte transformation capability(P>0.05).Conclusion Boxthorn seed oil and flaxseed oil had positive effects on mice immune function.