To evaluate the antimicrobial resistance of Enterococcus spp. using samples from the environment and retailed raw pork from free markets in Beijing. Methods The susceptibility of 86 isolated Enterococcus spp. to 10 antibiotics was determined by broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute). Results In all 86 isolates tested, 67.44% (58/86), 54.65% (47/86), 38.37% (33/86), 26.74% (23/86), 23.26% (20/86) and 20.93% (18/86) of Enterococcus spp. were resistant to tetracycline, erythromycin, ciprofloxacin, high-level streptomycin, high-level gentamicin, and chloramphenicol, respectively. It should be pointed out that 30 (34.88%) strains of Enterococcus spp. were resistant to more than 3 antibiotics, and 22 spectrums of antimicrobial susceptibility were observed. All Enterococcus spp. were susceptible to ampicillin, penicillin and vancomycin. According to the antibiotic profiles analysis, Enterococcus spp. isolated from different sources had significant difference on the antibiotic profiles. Moreover, daptomycin-resistant Enterococcus spp. from food-related samples was first reported in China.Conclusion The multi-drug resistance of Enterococcus spp. isolated from raw pork and environmental samples of the free markets was very serious, and their antibiotic profiles presented significant difference. It was the first time to isolate a daptomycin-resistant Enterococcus spp. from food. Therefore, close attention should be paid to drug resistance surveillance.