To establish a TaqMan-based real-time PCR approach to determine raccoon dog meat.Methods TaqMan primers and probe sets were designed by mitochondrial Cytb gene of raccoon dog species and PCR system was optimized to achieve real-time PCR and quick detection of products. Results This method had good specificity, and the detection limit was 10-4 ng DNA of raccoon dog.Conclusion This method could well meet the requirements of actual food testing.