To establish a method of rapid, sensitive, and accurate flow injection analysis (FIA) for the determination of cyanide in liquor.Methods After the sample being alkaline hydrolyzed by 2 g/L NaOH, the cyanide in liquor was determined directly by LACHAT QC8500 FIA under the optimized conditions. Results The matrix interference in liquor was successfully reduced, and the accuracy and sensitivity were improved. A significant linear correlation (r=0.999 9) existed within the range of 0.50-500 μg/L. The detection limit of the instrumental method was 0.17 μg/L. The recoveries of spiking samples and the relative standard deviations were 87.3%-97.9% and 2.2%-3.7% respectively.Conclusion The method is simple, sensitive, accurate for the determination of cyanide in different kinds of liquor.