To establish a method for determination of 6 plant growth regulator residues in fruits by liquid chromatography-tandem mass spectrometry using the isotope internal standard.Methods The sample was extracted by acetic acid in acetonitrile and homogenized. After dehydration reagent treatment, the sample was centrifugated and cleaned-up by dispersive solid phase extraction tube. The supernatant was detected by vortex mixing and centrifugation. The conditions of elution for mobile phase and analysis for mass spectrometry were optimized. Results The recovery test of the spiked sample was used with 3 concentrations, 0.6,0 and 50 μg/kg. The spiked sample recoveries were at 80.1%-115.0% and the relative standard deviations of detection were at 4.3%-9.9%. The limits of quantification and detection were 0.2-4.0 and 0.06-1.5 μg/kg.Conclusion The method is simple, accurate, sensitive and it can meet the determination requirements of pesticide residues in foods.