To study the subtyping, antimicrobial resistances and molecular characteristics of Listeria monocytogenes isolated from patients in Beijing.Methods 32 strains isolated from patients from 2013 to 2015 were characterized by serotyping with PCR, pulsed field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Results 32 Listeria monocytogenes isolates included 4 serotypes of 1/2b (17 strains), 1/2a (12 strains), 4b (2 strains) and 1/2c (1 strains). Digested by Asc I, 23 PFGE patterns were identified and divided into 4 clusters. The PFGE patterns of the same Listeria serotype were in the same cluster. The consistency of the two classification methods was 93.75% (30/32). All strains were sensitive to penicillin, ampicillin, sulfamethoxazole and erythromycin. Most of the strains had low MIC values to the other 8 kinds of antibiotics, but a few strains had high MIC values to oxacillin and tetracycline, even to 32 or 64 μg/ml.Conclusion The dominant serotypes of Listeria monocytogenes isolated from patients in Beijing were 1/2b and 1/2a. There were some correlations between serotypes and PFGE patterns. Resistance rate was low, but more attention should be paid to the resistance trend monitoring.