To analyze the virulence related-genes and pulsed-field gel electrophoresis (PFGE) characteristics of Vibrio vulnificus isolates in Guangzhou, and to establish regional PFGE database which would support molecular epidemiology of Vibrio vulnificus and traceability of foodborne disease.Methods 68 Vibrio vulnificus strains were characterized by the virulence related-genes and PFGE typing. Finger printing of each strain was analyzed by BioNumerics. Results All Vibrio vulnificus strains were positive for hemolysis A gene (vvhA).Five genetypes were detected, they were CB, EA, CAB, CA and EB type. 42 BT1 nucleic acid positive strain were detected. The 68 strains of Vibrio vulnificus were divided into 52 PFGE patterns.Conclusion The PFGE pattern of Vibrio vulnificus isolated from seafood was diversed, and CB type was the predominant virulence related-gene. It is necessary to strengthen the detection of Vibrio vulnificus in marine products to prevent the outbreak of Vibrio vulnificus infection.