A method for simultaneous determination of vitamin A, D and E in function foods by a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established.Methods Vitamins in the sample were saponified in the protection of nitrogen. After concentrated by rotary evaporation, and then dissolved by mobile phase, samples were determined by using HPLC-MS/MS selective reaction monitoring (SRM) determination of positive ion mode. Results The linear ranges were 10-1 000,2-100,1-100,5-2 000,5-500,5-500 and 5-500 μg/L for vitamin A, vitamin D₂, vitamin D₃, α-vitamin E, β-vitamin E, γ-vitamin E and δ-vitamin E respectively. The coefficient of variance of the method was in the range of 1.3%-5.5% (n=6). The average recovery in function foods was in the range of 90.4%-105.0%. Detection limits of seven vitamins were as follows, 2.0 μg/kg for vitamin A, 2.0 μg/kg for vitamin D₂, 0.2 μg/kg for vitamin D₃,0.2 μg/kg for α-vitamin E, 0.5 μg/kg for β-vitamin E, 0.5 μg/kg for γ-vitamin E, and 1.0 μg/kg for δ-vitamin E.Conclusion The method could be used for the qualitative and quantitative analysis of vitamin A, D and E in function foods. Precision, recovery rate and detection limit of the method could meet the practical requirements. It could be used for daily monitoring of vitamin A, D and E content in function foods or in other foods.