This paper reports an ultra performance liquid chromatography (UPLC) method for determination of 4 kinds of phytosterols (brassicasterol, stigmasterol, campesterol and β-sitosterol) in edible vegetable oil.Methods Edible vegetable oil samples were completely saponificated by glacial acetic acid, pH was adjusted to 6.0-7.5, then used ethanol to constant volume and freezing centrifuged to harvest supernatant. The analytical separation was performed on an Endoavorsil C₁₈ (100 mm×2.1 mm, 1.8 μm) chromatographic column with the isocratic elution using methyl alcohol and detected by photo-diode array detector at 205 nm. Results The linearity ranges were 20-200 μg/ml for β-sitosterol, stigmasterol and brassicasterol and 10-100 μg/ml for campesterol. The limits of detection were 23.2 μg/g for brassicasterol, 18.8 μg/g for stigmasterol, 18.6 μg/g for campesterol, and 23.8 μg/g for β-sitosterol. The quantitation limits were 77.5 μg/g for brassicasterol, 62.8 μg/g for stigmasterol, 62.2 μg/g for campesterol, and 79.4 μg/g for β-sitosterol. The average recoveries of spiked samples were 84.7%-100.7% and the RSD of method (n=6) was less than 3%.Conclusion The method was steady, simple, accurate and sensitive. It could be used to simultaneous detect 4 kinds of phytosterols in edible vegetable oil.