To evaluate the PetrifilmTM Salmonella express system of 3MTM company following the standards and specifications.Methods The inclusiveness and exclusivity of the PetrifilmTM Salmonella express plate were evaluated using 64 Salmonella strains of 53 serotypes and 30 non-Salmonella strains. Different Salmonella detection systems were compared and evaluated by parallel tests of a wide range of food samples inoculated with Salmonella with the GB method. Results The growth rates of 64 Salmonella strains on four selective media were significantly different (P<0.05), and the growth rate of 30 non-Salmonella also showed difference. Using different Salmonella selective enrichment broth-selective agar combination, the detection rate showed significant difference at 10-1 cfu/25 g level (P<0.05),and the highest detection rate was found by using RV(R10)-3M express plate. The using of two different selective enrichment broths and two selective agars (such as the GB method) could significantly improve the detection rate at 10-¹cfu/25 g level in food samples (P<0.05).Conclusion The PetrifilmTM Salmonella express system of 3MTM company was a rapid,convenient Salmonella detection kit and should be promoted in food microbiogy testing labs since it showed the same level of accuracy with the GB method in a wide range of food samples.