To compare the different effects of lentinan, pachymaran, tremella polysaccharide and their complex on cellular immunity of immuno-suppressed mice.Methods The function of T cells was detected by delayed type hypersensitivity (DTH) test, and the quantity of cytokines such as IL-2 and IL-6 in serum were detected using Luminex liquid protein microarray analysis system. The ratio and subtype of cells were detected by flow cytometry (FCM), and the proliferation of T cells was determined by using a colorimetric method with MTT. Results The ear swelling tends to be normal compared with the model group (P<0.05), and the lentinan group and polysaccharides complex group had the most significant difference (P<0.05). The tremella polysaccharide and polysaccharide complex could upregulated the level of IL-6 in serum of immuno-suppressed mice (P<0.05). All the polysaccharides had the tendency of enhancing the ratio of regulatory T cells (Tregs) in blood, the difference in tremella polysaccharide and polysaccharide complex had statistical significance comparing with model group(P<0.05). All test samples had function of restoring the ratio of Th1/Th2 cells in peripheral blood in immuno-suppressed mice (P<0.05), the function of polysaccharide complex was the most significant among them. All four polysaccharides had the tendency of restoring the ratio of splenic T and B cells in immuno-suppressive mice, the most obvious trend of which was polysaccharide complex. All the four polysaccharides had the tendency of promoting proliferation of splenic T cells, the difference of lentinan and polysaccharide complex was statistically significant comparing to the model group (P<0.05).Conclusion Lentinan, pachymaran, tremella polysaccharide and their complex could restore the cellular immunity in immuno-suppressed mice, and the complex was the best.