Using pulsed field gel electrophoresis for the separation of food poisoning strain for homology analysis, and providing a basis to find out the cause and traceability.Methods Samples from patients, food processing and leftovers were collected, isolated, identified and PFGE and antibiotic resistance analysis was performed.Results Four Salmonella Newport strains were detected in 13 samples, which were from the patient, the cooker, the remaining beef, and remaining duck. The PFGE patterns of the 4 strains were identical with the homology of 100%. 4 isolates had to the same resistance spectrum.Conclusion The food poisoning was caused by Salmonella Newport. PFGE typing techniques revealed epidemiological link between the strains, providing evidence for molecular epidemiological analysis and traceability of events.