To study the contamination and distribution of Vibrio parahemolyticus, Vibrio alginolyticu and Vibrio vulnificus in fish, water and underwater deposit from farming, saling and catering of freshwater fish in inland cities in China.Methods All monitoring sites were in Guangxi, Henan, Hubei, Shandong, Sichuan and Zhejiang Provinces. Three kinds of halophilic Vibrio from different samples were analyzed by conventional method such as culturing, isolating and biochemical identification. Results The occurrence was 1.89% and 5.81% for Vibrio parahemolyticus isolated from farming water and freshwater fish, 1.89% and 4.98% for Vibrio alginolyticu, and 1.14% and 0.83% for Vibrio vulnificus. The occurrence increased rapidly in markets from temporary keeping water and freshwater fish with 18.47% and 14.93% for Vibrio parahemolyticus, 10.83% and 9.70% for Vibrio alginolyticu, and 3.18% and 3.36% for Vibrio vulnificus. The occurrence of three kinds of halophilic Vibrio isolated from freshwater fish was basically identical between markets and the restaurants.Conclusion The marine Vibrio, Vibrio parahemolyticus, Vibrio alginolyticu andVibrio vulnificus, have been isolated from farming, saling and catering of freshwater fish in inland cities, and it should call attention to the safety of the food chain.