A method was developed for the simultaneous determination of four eugenol derivatives residues(eugenol, isoeugenol, methyl eugenol, methyl isoeugenol) in aquatic products by solid-phase extraction-high performance liquid chromatography with fluorescence detection.Methods The samples were extracted by acetonitrile. The extracted solutions were cleaned up by neutral alumina coupled with C₁₈ solid-phase extraction column. The target compounds were separated on a reversed phase C₁₈ column using methanol-acetonitrile and 1% acetic acid as mobile phase, detected by the fluorescence detector at an excitation wavelength of 280 nm and an emission wavelength of 320 nm. External standard method was applied for quantitative analysis. Results The calibration curves of four eugenol derivatives showed good linearity within their respective linear ranges and high correlation coefficients (r>0.999). The limits of detection and the limits of qualification were 3.0-6.0 and 10.0-20.0 μg/kg, respectively.The average recoveries were in the range of 74.7%-103.0% with the relative standard deviations (RSDs) of 2.6%-8.1%. Conclusion The method was simple, highly sensitive and practical. It was suitable for the determination of eugenol, isoeugenol, methyl eugenol and methyl isoeugenol residues in aquatic products.