Meat identification based on Cytb gene sequences for the market meat products
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(College of Life Science and Technology,Southwest University for Nationalities,Sichuan Chengdu 610041, China)

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    Abstract:

    To explore the identification of the meat and meat products based on Cytb gene.Methods A total of 21 samples were collected randomly. DNAs were extracted by CTAB method. Conventional PCR technique was applied to amplify Cytb genes and the amplified PCR products were sequenced. DNAMAN software was used to align the Cytb sequences. Results CTAB method was suitable for DNA extraction in meat products. The DNA sequencing and alignment results indicated that 9 beef samples were consistent with the label, and one was pork; one mutton sample was consistent with label, another was detected as pork; one pork sample was consistent with label, and the other was detected as chicken; one chicken sample was consistent with label; 2 yak samples were consistent with label, and four were detected as water buffalo meats.Conclusion Sequences alignment method based on Cytb gene was suitable for the market meat products identification. Moreover, meat adulteration was very serious.

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MA Yi-sa-lan, LV Ming-xing, TANG Jun-ni, CHEN Juan, ZHANG Rong, LONG Hu. Meat identification based on Cytb gene sequences for the market meat products[J].中国食品卫生杂志,2016,28(1):48-51.

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History
  • Received:December 01,2015
  • Revised:
  • Adopted:
  • Online: February 19,2016
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