To investigate the pathogen of a food poisoning accident and provide scientific evidence to identify the cause.Methods Isolation, identification and toxicological assessment of toxic agents were carried out according to the national standard GB/T 4789.29-2003, bongkrekic acid was detected by liquid-plasma chromatography techniques according to GB/T 11675-2003. Results By VITEK 2 COMPACTR and Ribo-Printer microbial characterization system, Burkholderia gladioli was identified in 3 of 4 food samples, but all of the 4 food samples were confirmed to be contaminated by Pseudomonas cocovenenans subsp.Farino. A positive toxicological result was found in mice acute oral toxicity test.Conclusion The food poisoning accident was associated with ingestion of foods contaminated by Pseudomonas cocovenenans subsp.Farino.