To investigate the molecular epidemiological relationship of Salmonella enteritidis strains isolated from 2 food poisoning events occurred successively in the same location.Methods Pathogen isolation and culture was conducted according to GB 4789.4-2010. The phenotype of isolates were identified. VITEK-Ⅱ compact automatic bacteria identification system was used for the antibiotic resistance analysis. Pulsed field gel electrophoresis (PFGE) was used for the molecular subtyping and epidemiological characteristics analysis. Results 23 strains of Salmonella were isolated, among which 17 strains were isolated from anal swab specimens of 25 patients, 2 strains from 8 employees, 3 strains from reserved sample of food and 1 strain from kitchenware. Serum antigen type were (9∶m∶-). The result of the biological characteristic and antibiotic resistance analysis were basically the same. PFGE pattern were the same.Conclusion Epidemiological investigation, pathogen detection and molecular typing indicated that these two cases of foodborne outbreaks were caused by the same source of Salmonella enteritidis contamination.