To investigate the subspecies, phenotypic and genetic characterization of Cronobacter isdates from infant food in 2010.Methods All isolates were reactivated, then suspectable stains were identified preliminarily by two methods, PCR amplifying ompA gene and API 20E. To confirm these stains, full 16S rRNA gene sequences were obtained, and made BLASTN on GeneBank database. The identified strains were streaked onto DFI chromogenic agar and TSA to observe phenotype and yellow pigment production. Biochemical tests were conducted and assigned to biogroups to determine their species.Results Nine strains were identified as Cronobacter spp. These strains were assigned to 5 biogroups, and belonged to 4 species. The majority of strains were Cronobacter sakazakii, and three strains was identified as C.malonaticus, C.muytjensii and C.dublinensis respectively. 7 strains were identified as Cronobacter by API 20E, yet ompA gene amplification of all strains was positive.Conclusion Cronobacter isolated from infant food were diversed in biochemical phenotype and species. There was a risk of Cronobacter in infant food from supermarkets. There was limitation to identify the bacteria only by biochemical phenotype, and supplementary molecular biology methods was necessary.