To study biological sealed sample during 2009-2014 in microbiology laboratory of the LNCDC (Liaoning Centers for Disease Control and Defense) and LNIFC (Liaoning Institute of Food Cntrol) and conclude inspection process and the key point of this assessment of biological sealed sample.Methods Testing analysis of biological sealed sample for 5 years according to the national standard GB 4789-2010 National Food Safety Standards of Food Microbiology Inspection. Results The assessment results are good or satisfied for the results of 8 times examination and 13 projects involved in.Conclusion The assessment of biological sealed sample biological sealed sample examination should focus on inspection process and the key points. Participate of biological sealed sample biological sealed sample examination are helpful for the promotion of laboratory work ability, ensuring the daily test results accurate and reliable.