To investigate the status of knowledge,attitude and practice on food safety among food employees engaged in high-speed rail diner, and then provide scientific basis for education and training on food safety among food employees.Methods A questionnaire survey was conducted among 542 food waiters engaged in high-speed rail diner. The data were analyzed by the soft of SPSS 13.0. Results The total average mark on food safety knowledge of employees was 5.79±1.67 (full mark was 10), and 44.0% of the respondents got more than 6, and 13.7% of the respondents got more than 8.416 respondents (96.7%) hoped to get more vocational knowledge about food safety. The total correct behavior rate of food safety was 78.6%. The higher educational level of the respondents got the higher mark of food safety knowledge, and the differences between the groups divided by educational level were statistically significant (P＜0.01). In the groups divided by age, the food safety knowledge mark and correct behavior rate of the informants less than 20 years old were both much lower than those in other groups (P＜0.01). As far as the working age be concerned, the food safety knowledge mark and correct behavior rate of the informants whose working age less than 1 year were both much lower than those in other groups (P＜0.01).Conclusion The food employees engaged in high-speed rail diner had some knowledge about food safety, but still had deficiencies. Their attitudes on getting more vocational knowledge about food safety were active. It was necessary to carry on food safety interventions to help the employees to obtain more food safety knowledge and to form correct behavior habits, especially for those of low educational level and short working age.