To optimize the ISO 6579∶2002 method using modified semisolid tetrathionate (MSTT) medium which was introduced for preliminary screening of Salmonella in poultry products.Methods MSTT medium was used for motility enrichment of Salmonella. It was loaded by capillary tube which was inserted into liquid medium (pre-enrichment medium or enrichment medium). Therefore, the semisolid medium and liquid medium were connected, and motility enrichment would proceed simultaneously with pre-enrichment or with enrichment. The optimized ISO 6579.2002 method using MSTT medium was evaluated with pure strains and actual contaminated poultry samples. Results The method could detect 98% (126/129) of pure strains, and could detect 92% (35/38) of the contaminated poultry samples, while ISO 6579.2002 method could detect 87% (33/38) of the contaminated poultry samples. Comparing to ISO 6579.2002 method, 72% (264/367) of the negative samples did not need further isolation and identification after using MSTT medium. Conclusion The optimized ISO 6579.2002 method using MSTT medium is suitable for Salmonella detection in poultry, and could save labour and resources.