To understand the prevalence of genes conferring aminoglycoside resistance in foodborne strains of Proteus.Methods 124 strains of Proteus were collected from food samples of markets in Shijiazhuang from 2011 to 2014, which resisted toamikacin (AK) or/and gentamicin (CN). 6 kinds of aminoglycoside modifying enzyme genes (AMEs) and 3 kinds of 16S rRNA methylase genes were analyzed by PCR. Results The prevelence of the AMEs genes , aacC2, aacA4, aadA1 and aphA6,was 95.2% (118/124), 80.6%(100/124), 73.4% (91/124) and 5.6%(7/124) respectively. The prevenlence of 16S rRNA methylase gene rmtB was 87.1% (108/124). Other genes conferring aminoglycoside resistance, such as aacC1, aadB, armA and rmtC gene were not detected. Conclusion The spread of AMEs gene aacC2 and 16S rRNA methylase gene rmtB plays an important role in aminoglycosides resistance of foodborne Proteus.