To acquire original data of Salmonella serotype distribution during pig and poultry slaughter processes in Shandong Province, and to provide data for the risk assessment of animal products.Methods Salmonella was isolated from slaughterhouses in Shandong Province from September to December 2014. xMAP Salmonella serotyping assay kit was used to detect the genes of somatic O antigen, motility H antigen and additional target (AT) gene. Salmonella serotypes were identified by xMAP Salmonella serotyping assay software throught gene information. Results A total of 299 Salmonella isolates were divided into 29 serotypes and 36 isolates could not be typed. S.Derby (24.8%,83/335) and S.Enteritidis (15.2%,51/335) were the major serotypes in pig and poultry slaughter processes in Shandong Province. S.Derby, S.Thompson and S.Agona were detected both in pig and poultry slaughter processes; S.Derby (39.8%,64/161), S.typhimurium (19.3%,31/161) and S.Thompson(13.7%,22/161) were themajor serotypes in pig slaughter process; S.enteritidis (28.7%, 50/174), S.Indiana (12.1%, 21/174) and S.Derby (10.9%, 19/174) were the major serotypes in poultry slaughter process. Salmonella serotypes varied in various areas of Shandong Province. In the pig slaughter processes, S.Derby was the major serotype except the middle of Shandong Province; In the poultry slaughterhouses, S.enteritidis was the major serotype in the east and west of Shandong Province, S.Derby was the leading serotype in the northeast; S.Thompson was the most prevalent in the middle of Shandong Province.Conclusion There were various Salmonella serotypes at slaughterhouses in Shandong Province, and slaughter processes supervision and control should be strengthened to decrease the risk of Salmonella contamination in animal products.