This paper reports a new, simple and rapid method to determine histamine, cadaverine, putrescine, tyramine, tryptamine, spermine, spermidine and β-phenylethylamine in wine without sample pretreatment.Methods Following automatic online derivatization with a mixed solution of cysteamine and o-phthaldialdehyde, eight kinds of biogenic amines in wine were separated on a high performance liquid chromatography instrument with a C₁₈ column. Results The linear range of the proposed method was validated in the range 0.25-10 mg/L. The relative standard deviation was less than 5%, and the intermediate precision ranged from 2.5 to 6.3%. The recoveries varied from 91.2% to 104.1%, and the detection limits varied from 20 to 100 μg/L for the eight kinds of biogenic amines.Conclusion The method is able to accurately determine the content of eight kinds of biogenic amines in wine with good reproducibility, accurate quantization and without sample pretreatment.