Analysis and comparison of crude fat expression effect of five kinds of WRI1 transgenic rice.Methods The coconut WRI1 gene (regulation genesof fatty acid synthesis ) was introduced into japonica rice zhonghua11, and 5 transgenic plants, ZZWR1, ZZWR2, ZZWR3, ZZWR4 and ZZWR5 were obtained. The crude fat of the transgenic rice and control rice was extracted by Soxhlet extraction method and determined qualitatively and quantitatively using GC-MS method.Results Compared with the control rice which contained 2.59% crude fat, the contents of ZZWR1, ZZWR2, ZZWR4 were from 2.62% to 2.77%, which was significantly higher than the control rice. ZZWR4 was the highest with RSD of 0.96%. Palmitic acid methyl ester, methyl stearate and methyl oleate were quantified by GC-MS , and methyl oleate was the highest.Conclusion Coconut WRI1 gene introduced into rice could improve rice fat content. The different insertion sites of WRI1 gene may influence the original fatty acid expression. ZZWR4 insertion site had better expression.