To assess the potential acute and chronic health risk of oral exposure to methanol in tequila in Chinese adult drinkers.Methods Oral exposure threshold limit values in human were chosen by toxicity data. A conservative estimation was used. Instead of high percentile tequila consumption data, high percentile distilled spirit consumption data combined with 3.0 g/L (100% ethanol) methanol in tequila as the maximum concentration were employed for acute exposure assessment. Instead of average tequila consumption data, average distilled spirit consumption data combined with 1.8 g/L (100% ethanol) methanol in tequila reported by reference published were employed for chronic exposure assessment. Results The estimated single intake of methanol from tequila was 11.25 mg/kg BW which was lower than single oral exposure dose (20 mg/kg BW). The estimated intake of methanol from tequila was 1.69 mg/kg BW which was lower than the recommended reference dose of 2.0 mg/kg BW.Conclusion Acute and chronic health risk of methanol from Tequila was ignorable.