To investigate the contamination of aflatoxin B₁ in food and oil in Guangzhou, and provide the basic data of dietary intakes of aflatoxin B₁ for food safety assessment.Methods The samples of seven kinds of food including rice, wheat flour, peanut and corn oil, peanut, corn flour, fried food and soybean from ten regions were collected randomly from farmer's markets, supermarkets, wholesale markets, and catering units. The national standard detection method for aflatoxin B₁ (ELISA) was taken to detect the content of aflatoxin B₁. Results 260 samples were detected aflatoxin B₁ and the detection rate was 31.71%, and the farmer's markets had the highest detection rate. The detection range was 0.025～39.300 μg/kg, with the mean value of 2.675 μg/kg and the median of 2.5 μg/kg. The overall qualified rate was 98.66%.Conclusion The overall level of aflatoxin B₁ contamination in market food was low, but some foods such as vegetable oils (peanut, corn) should be more concerned.