Loop-mediated isothermal amplification (LAMP) method was developed to detect porcin, duck and sheep-derived materials in meat products (lamb chops, lamb meat roll, lamb shashlik).Methods A set of four specific primers (F3, B3, FIP, BIP) and two loop primers (FLP and BLP) were designed, respectively. Optimization reaction was confirmed, and two different measurement platforms were compared. 29 samples of meat products were collected from local markets and the detection of porcin, duck and sheep-derived materials was carried out.Results The optimized condition was 0.2 μmol/L each of F3 and B3, 1.6 μmol/L each of FIP and BIP, 0.8 μmol/L each of FLP and BLP, 1 mol/L betaine, 6 mmol/L Mg²⁺, 1.6 mmol/L dNTP. The limit of detection was up to 0.5% animal-derived materials in meat products under the platform of real time fluorescence and nake-eye inspection with SYBR Green I. The current results of LAMP obtained on the two measurement platforms were in good accordance. With the LAMP assay, meat products were successfully screened for porcin, duck and sheep-derived materials.Conclusion This method is a highly specific and sensitive detection system, and could be a very useful tool for animal components detection.