To investigate the molecular characteristics and antibiotic resistance of nontyphoidal Salmonella isolated from food in Beijing.Methods A total of 100 strains were isolated from foodborne pathogenic bacteria monitoring network in Beijing from 2004 to 2010, and were analyzed by PFGE and antimicrobial susceptibility test. Results The isolates were divided into 62 PFGE pattern, and each contains 1-11 strains. The result of antimicrobial susceptibility test showed that 55 strains were resistant to at least one antibiotic, including 15 multidrug resistant strains. The resistance rate to the eight antibiotics were nalidixic acid (40%), tetracycline (30%), chloramphenicol (15%), gentamicin (10%), trimethoprim/sulfamethoxazole (10%), ciprofloxacin (9%), cefoxitin (1%), and cefotaxime (0%).Conclusion PFGE profiles, antibiotic resistance patterns and serotypes of Salmonella showed high consistency. The antibiotic resistance of foodborne nontyphoidal Salmonella in Beijing was serious, and enhancing the joint monitoring of molecular subtyping and antibiotic resistance has significant importance.